The Effect of Antigenic Stimulation on Incorporation of Phosphate and Methionine into Proteins of Isolated Lymph Node Cells
نویسندگان
چکیده
Isolated lymph node cells incorporate inorganic orthophosphate into a protein fraction. The phosphorylated product is a phosphoprotein. The rate of phosphate incorporation into phosphoprotein was determined in cells isolated from regional lymph nodes at varying times after antigen injection. The rate was unaltered on the 3rd day, but was enhanced on the 4th day after injection. Parallel results were obtained with L-methionine incorporation into the same gross protein fraction. Possible relationships between antibody formation and the observed enhancement in phosphate incorporation into phosphoprotein are discussed.
منابع مشابه
T H E Effect of Antigenic Stimulation on Incorporation of Phosphate and Methionine into Proteins of Isolated Lymph Node Cells* by Milton
An important means for analyzing a number of basic immunological problems has been furnished by ~ e discovery that lymphoid ceils derived from immunized and sensitized donors will transfer to untreated recipients of the same species the immune and allergic reactivities exhibited by the donor (1, 2). Successful transfer among laboratory rodents has thus far only been accomplished consistently wi...
متن کاملT H E Effect of Antigenic Stimulation on Incorporation of Phosphate and Methionine into Proteins of Isolated Lymph Node Cells* by Milton
An important means for analyzing a number of basic immunological problems has been furnished by ~ e discovery that lymphoid ceils derived from immunized and sensitized donors will transfer to untreated recipients of the same species the immune and allergic reactivities exhibited by the donor (1, 2). Successful transfer among laboratory rodents has thus far only been accomplished consistently wi...
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عنوان ژورنال:
- The Journal of Experimental Medicine
دوره 110 شماره
صفحات -
تاریخ انتشار 1959